Mung bean nuclease is widely used in molecular biology for its ability to specifically digest single-stranded DNA and RNA. It is particularly valuable in the removal of single-stranded overhangs from DNA fragments, making it essential for creating blunt ends in cloning experiments. This enzyme is also employed in the mapping of RNA structures and the analysis of nucleic acid-protein interactions, as it can selectively degrade unpaired regions without affecting double-stranded areas. Additionally, mung bean nuclease is utilized in PCR cleanup protocols to eliminate primer dimers and other single-stranded artifacts, ensuring higher purity of amplified products. Its specificity and efficiency make it a critical tool in various genetic engineering and biotechnological applications.