Ribonuclease T1 from Aspergillus oryzae is widely used in molecular biology research for its ability to specifically cleave RNA at guanosine residues. This enzyme is particularly valuable in RNA sequencing and mapping, where it helps in determining the structure and sequence of RNA molecules. It is also employed in the study of RNA-protein interactions, as it can be used to digest unprotected RNA regions while leaving protein-bound regions intact. Additionally, Ribonuclease T1 is utilized in the preparation of RNA samples for various analytical techniques, such as gel electrophoresis and mass spectrometry, ensuring precise and accurate results. Its high specificity and efficiency make it a crucial tool in advancing our understanding of RNA biology and its role in cellular processes.