4-Cyanocinnamic Acid is widely used in the field of analytical chemistry, particularly in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. It serves as an effective matrix for the analysis of peptides, proteins, and other biomolecules due to its ability to absorb UV light and facilitate the ionization of analytes. This compound is also utilized in organic synthesis as a building block for the preparation of more complex molecules, including pharmaceuticals and agrochemicals. Its cyanide and carboxylic acid functional groups make it a versatile intermediate in various chemical reactions, such as cycloadditions and condensations. Additionally, 4-Cyanocinnamic Acid finds applications in material science, where it is used in the development of organic electronic materials and nonlinear optical materials due to its conjugated system and electron-withdrawing properties.