N-Octyl-β-D-glucopyranoside is widely used as a non-ionic detergent in biochemical research. Its ability to solubilize membrane proteins without denaturing them makes it a valuable tool for studying protein structures and functions. It is often employed in the purification and crystallization of membrane proteins, aiding in the elucidation of their 3D structures.

In the field of biotechnology, it is utilized in the preparation of liposomes and other lipid-based drug delivery systems. Its mild surfactant properties help in stabilizing these systems, enhancing their efficiency in targeted drug delivery. Additionally, it is used in cell lysis procedures to gently disrupt cell membranes, facilitating the extraction of intracellular components while preserving their integrity.

N-Octyl-β-D-glucopyranoside also finds applications in the cosmetic industry, where it is used as an emulsifier in formulations. Its ability to stabilize mixtures of oil and water makes it suitable for use in creams, lotions, and other personal care products. Furthermore, it is employed in various diagnostic assays and laboratory techniques, contributing to the accuracy and reliability of experimental results.