Triton X-114 is widely used as a nonionic surfactant in various biological and biochemical applications. It is particularly effective in the extraction and purification of membrane proteins due to its ability to solubilize lipid bilayers without denaturing the proteins. This property makes it a valuable tool in research involving cell membrane studies and protein isolation.

In biotechnology, Triton X-114 is utilized in phase separation techniques, where it helps separate hydrophilic and hydrophobic molecules. This is especially useful in the purification of recombinant proteins and in the study of protein-protein interactions. Its temperature-dependent cloud point allows for easy separation of phases, simplifying the purification process.

Additionally, Triton X-114 is employed in diagnostic assays and immunoprecipitation protocols, where it aids in the solubilization of antigens and enhances antibody binding. Its mild detergent properties ensure that the biological activity of proteins is preserved, making it suitable for sensitive applications.

In industrial settings, Triton X-114 is used as an emulsifying agent in formulations of cleaning products, cosmetics, and pharmaceuticals. Its ability to stabilize emulsions and improve the solubility of active ingredients contributes to the effectiveness of these products.