Triton X-114 is widely used in biochemistry and molecular biology for the extraction and purification of membrane proteins. Its ability to form micelles makes it effective in solubilizing hydrophobic proteins while maintaining their functionality. This surfactant is particularly useful in temperature-induced phase separation techniques, where it helps isolate membrane proteins from aqueous solutions by partitioning them into the detergent-rich phase. Additionally, Triton X-114 is employed in cell lysis procedures to disrupt cell membranes and release intracellular components. It is also utilized in the study of protein-protein interactions and in the preparation of samples for electrophoresis and chromatography. Its mild nature and efficiency in solubilizing membrane-bound proteins make it a valuable tool in various research applications.