NP40 Lysis Buffer with Glycerol, 2X is widely used in molecular biology and biochemistry for cell lysis and protein extraction. Its non-ionic detergent, NP-40, effectively disrupts cell membranes while preserving protein structure and function. The inclusion of glycerol stabilizes proteins and prevents their denaturation during the extraction process. This buffer is particularly useful for isolating cytoplasmic and nuclear proteins from cultured cells or tissues. It is also employed in immunoprecipitation assays, where maintaining protein integrity is crucial for accurate results. Additionally, the buffer's compatibility with downstream applications, such as Western blotting and enzyme activity assays, makes it a versatile tool in protein research. Its 2X concentration allows for easy dilution to achieve the desired working strength, ensuring consistent performance across experiments.