NP40 Lysis Buffer is widely used in molecular biology and biochemistry for cell lysis and protein extraction. It effectively disrupts cell membranes while maintaining the integrity of intracellular proteins, making it ideal for isolating proteins from cells or tissues. The buffer is commonly employed in immunoprecipitation, Western blotting, and other protein analysis techniques. Its mild detergent properties allow for the solubilization of membrane-associated proteins without denaturing them, ensuring their functionality for downstream applications. Additionally, it is often used in preparing cell lysates for studying protein-protein interactions, enzyme activity assays, and other biochemical analyses. Its versatility and efficiency make it a staple in laboratories focused on protein research.