NP40 Permeabilizing Solution in TBS is widely used in biological research for cell membrane permeabilization. It is particularly effective in immunofluorescence and immunohistochemistry protocols, where it allows antibodies and other detection reagents to penetrate cells and access intracellular antigens. This solution is also utilized in protein extraction procedures, as it helps solubilize membrane-bound proteins while maintaining their native structure. Additionally, it is employed in flow cytometry to enhance the staining of intracellular markers. Its mild detergent properties make it suitable for preserving cell morphology and protein integrity during permeabilization processes.