NP40 Lysis Buffer is widely used in molecular biology and biochemistry for cell lysis and protein extraction. It is particularly effective for isolating proteins from cell membranes and cytoplasm due to its ability to disrupt lipid bilayers while maintaining protein integrity. This buffer is commonly employed in immunoprecipitation, Western blotting, and enzyme activity assays, as it solubilizes proteins without denaturing them. It is also suitable for extracting nuclear and cytoplasmic proteins from cultured cells, making it a versatile tool in research laboratories. Additionally, NP40 Lysis Buffer is often used in studying protein-protein interactions and in preparing samples for downstream analytical techniques.