NP40 Lysis Buffer, Low Salt, is widely used in molecular biology and biochemistry for the gentle extraction of proteins from cells. Its low salt concentration helps maintain protein stability and prevents denaturation, making it ideal for studying protein-protein interactions and enzyme activities. This buffer is particularly effective for lysing cells while preserving the integrity of cellular organelles and membranes. It is commonly employed in immunoprecipitation (IP) and co-immunoprecipitation (Co-IP) experiments to isolate specific proteins or protein complexes. Additionally, it is used in preparing samples for Western blotting, ensuring that proteins remain in their native state for accurate detection and analysis. Its mild detergent properties make it suitable for extracting cytoplasmic proteins without disrupting nuclear membranes.