Tridecyl β-D-maltoside is widely used as a non-ionic surfactant in biochemical and biophysical research, particularly for the solubilization and stabilization of membrane proteins. Its mild detergency helps maintain the native structure and function of proteins during extraction and purification processes. It is especially effective in membrane protein crystallization due to its ability to form micelles with favorable properties for promoting crystal lattice formation. The compound is also employed in studies involving liposomes and vesicle formation, where consistent micelle size and low critical micelle concentration contribute to reproducible experimental outcomes. Its high purity and low denaturing potential make it suitable for use in techniques such as circular dichroism, NMR, and functional assays requiring active membrane proteins.