L-701324 is primarily used in research settings as a selective antagonist for the glycine site of the NMDA (N-methyl-D-aspartate) receptor. It is particularly valuable in studies focused on understanding the role of NMDA receptors in neurological processes. Researchers utilize it to investigate conditions such as stroke, epilepsy, and neurodegenerative diseases like Alzheimer’s, where NMDA receptor dysfunction is implicated. By blocking the glycine site, L-701324 helps elucidate the mechanisms of excitotoxicity, which is the process by which nerve cells are damaged or killed by excessive stimulation. Additionally, it aids in exploring potential therapeutic strategies for neuroprotection and managing psychiatric disorders linked to NMDA receptor activity. Its specificity makes it a useful tool in pharmacological and neurobiological research.