ML-261 is primarily utilized in biochemical research as a selective inhibitor of the Nod-like receptor protein 3 (NLRP3) inflammasome. Its application is significant in studying inflammatory diseases, as it helps in understanding the mechanisms underlying conditions like Alzheimer's disease, atherosclerosis, and type 2 diabetes. By inhibiting NLRP3, ML-261 provides insights into the role of this inflammasome in immune responses and cellular processes. Researchers also use it to explore potential therapeutic strategies for diseases characterized by excessive inflammation. Additionally, ML-261 serves as a valuable tool in pharmacological studies to identify and validate new targets for anti-inflammatory drug development.