N-Nitroso-methyl ethylamine is primarily used in research settings as a model compound for studying nitrosamine chemistry and their biological effects. It serves as a tool in toxicology and carcinogenesis studies due to its ability to alkylate DNA, helping scientists understand the mechanisms of chemical-induced cancer. Its reactivity makes it useful in probing metabolic activation pathways of nitrosamines in liver enzymes, particularly cytochrome P450 systems. Due to its instability and high toxicity, it is not used in industrial or commercial applications and is handled only under controlled laboratory conditions for scientific investigation.