N-Nitrososarcosine is primarily used in research settings to study the formation and effects of nitrosamines, which are compounds of significant interest due to their potential carcinogenic properties. It serves as a model compound to understand the mechanisms of nitrosation reactions, which are crucial in assessing the risks associated with nitrite-preserved foods and other environmental exposures. Additionally, it is employed in biochemical studies to investigate the interactions between nitrosamines and biological molecules, providing insights into DNA damage and mutagenesis processes. This chemical is also utilized in the development of analytical methods for detecting and quantifying nitrosamines in various matrices, contributing to food safety and environmental monitoring efforts.