NOS-IN-1 is primarily used in research settings to study the role of nitric oxide synthase (NOS) enzymes in various biological processes. It serves as a selective inhibitor, helping scientists understand the mechanisms of NOS in conditions like inflammation, neurodegenerative diseases, and cardiovascular disorders. This compound is particularly valuable in experimental models to explore potential therapeutic strategies by modulating nitric oxide production, which plays a critical role in cellular signaling and homeostasis. Its application extends to drug discovery efforts aimed at developing treatments for diseases where NOS activity is dysregulated.