Used as a nitric oxide (NO) donor in biochemical and pharmacological research, particularly in studies involving smooth muscle relaxation, vascular tone regulation, and neurotransmission. It allows controlled release of NO under physiological conditions, making it valuable for investigating NO-mediated signaling pathways in cells and tissues. Commonly applied in assays related to cyclic GMP production, enzyme activity modulation, and cellular response to oxidative stress. Its stability and specificity support its use in both in vitro and ex vivo experimental models.