PTIO is widely used as a scavenger for nitric oxide (NO) in biological and biochemical research. It helps in studying NO-mediated pathways by selectively reacting with NO to form a stable byproduct, allowing scientists to determine the role of nitric oxide in processes such as vasodilation, immune response, and neurotransmission. Due to its specificity, PTIO is valuable in experiments involving cellular signaling, oxidative stress, and inflammation. It is also employed in assays to confirm the involvement of NO in observed effects by blocking its activity. Additionally, PTIO has been explored in pharmacological studies for potential therapeutic applications where modulation of NO levels is required.