Used primarily in research settings as a fluorescent probe for detecting nitric oxide (NO) in biological systems. It reacts selectively with nitric oxide to form a stable, highly fluorescent compound, allowing scientists to visualize and quantify NO production in cells and tissues. This application is particularly valuable in studies related to vascular function, neurotransmission, and immune response, where nitric oxide acts as a key signaling molecule. Its sensitivity and selectivity make it a useful tool in assays aimed at understanding oxidative stress and inflammatory processes.