Thermopsidine is primarily used in scientific research, particularly in the study of ion channels and neuropharmacology. It acts as a potent agonist at certain nicotinic acetylcholine receptors, making it a valuable tool for investigating cholinergic signaling in the nervous system. Due to its structural similarity to other alkaloids with bioactive properties, thermopsidine is also studied for its potential effects on neuromuscular transmission and central nervous system activity. Its application extends to the development of pharmacological models for neurological disorders, where modulation of nicotinic receptors plays a key role. Additionally, it serves as a reference compound in receptor binding assays and electrophysiological experiments.