Norfluorocurarine is primarily used in scientific research as a pharmacological tool to study neuromuscular transmission. It acts as a competitive antagonist at nicotinic acetylcholine receptors, particularly at the neuromuscular junction, making it useful for investigating muscle paralysis mechanisms and receptor blocking effects. Due to its structural similarity to other curare alkaloids, it has been employed in experiments exploring the function of cholinergic systems in both peripheral and central nervous systems. Its application remains limited to laboratory settings, with no current therapeutic use in clinical medicine.