Used primarily in research settings as a cholinergic agonist to study acetylcholine receptors. It mimics the action of acetylcholine, making it valuable in neuropharmacology for investigating parasympathetic nervous system functions. Commonly employed in in vitro experiments to stimulate muscle contraction in smooth muscle tissues, such as intestinal and uterine preparations. Also utilized in studies assessing cholinergic signaling pathways and receptor subtype selectivity. Due to its methyl group modification, it exhibits increased metabolic stability compared to acetylcholine, allowing for prolonged activity in experimental models.