Used as a neuromuscular blocking agent in research settings, particularly in studies involving nicotinic acetylcholine receptors. It acts as a depolarizing agent, mimicking acetylcholine at the neuromuscular junction, leading to initial muscle contraction followed by paralysis due to receptor desensitization. Commonly employed in pharmacological experiments to investigate synaptic transmission and receptor dynamics. Also utilized in toxicology studies to assess cholinergic system responses and antidote efficacy. Its quaternary ammonium structure ensures limited blood-brain barrier penetration, making it suitable for peripheral nervous system studies.