Used primarily in neuroscience research as a cholinergic agonist to study acetylcholine receptors. It mimics the action of acetylcholine by binding to muscarinic and nicotinic receptors, allowing researchers to investigate signal transduction mechanisms in neurons and muscle cells. Due to its bromoacetyl group, it can also act as an irreversible receptor modifier, enabling covalent labeling of receptor sites for biochemical analysis. Commonly applied in studies examining neurotransmission, synaptic plasticity, and receptor function in both central and peripheral nervous systems.