Used in research to study cholinergic systems in the nervous system, particularly in neuroscience and pharmacology. Acts as a neurotransmitter mimic to activate acetylcholine receptors, including nicotinic and muscarinic types. Employed in experiments involving synaptic transmission, muscle contraction, and neuronal signaling. Also utilized in the development and testing of drugs targeting Alzheimer’s disease, myasthenia gravis, and other disorders related to cholinergic function. Commonly applied in in vitro preparations such as isolated tissue baths to examine receptor responses and drug effects.