Used extensively in biochemical research to study cellular respiration and energy production. It plays a critical role in assays measuring dehydrogenase enzyme activity, helping to understand metabolic pathways. In medical diagnostics, it is employed to detect and monitor diseases related to mitochondrial dysfunction. Additionally, it is utilized in the development of therapeutic agents targeting oxidative stress and aging-related conditions. In industrial applications, it is used in enzymatic synthesis for producing pharmaceuticals and fine chemicals.