This compound is widely used in biochemical research as a coenzyme in redox reactions. It plays a critical role in metabolic processes, particularly in glycolysis and the citric acid cycle, where it facilitates the transfer of electrons. In enzymatic assays, it serves as a reducing agent to study the activity of dehydrogenases and other enzymes. Additionally, it is employed in cell culture studies to support cellular respiration and energy production. Its reduced form is essential for maintaining redox balance in biological systems, making it valuable in studies related to oxidative stress and mitochondrial function.