BH3I-1 is primarily utilized in research settings as a selective inhibitor of the enzyme IκB kinase (IKK). Its application is significant in studying the NF-κB signaling pathway, which plays a crucial role in regulating immune responses, inflammation, and cell survival. By inhibiting IKK, BH3I-1 helps researchers understand the mechanisms underlying various diseases, including cancer, autoimmune disorders, and chronic inflammatory conditions. This compound is particularly valuable in preclinical studies aimed at developing targeted therapies for diseases where NF-κB signaling is dysregulated. Additionally, BH3I-1 is used in biochemical assays to explore the interactions between IKK and other cellular components, providing insights into potential drug targets. Its selective inhibition properties make it a useful tool for dissecting complex signaling networks in cellular and molecular biology research.