IKK-16 is primarily used in research settings to study the NF-κB signaling pathway, which plays a crucial role in inflammation, immune response, and cell survival. By inhibiting IκB kinase (IKK), this compound helps researchers understand the mechanisms of diseases like cancer, autoimmune disorders, and chronic inflammatory conditions. It is particularly valuable in preclinical studies to evaluate the potential therapeutic effects of targeting the NF-κB pathway. Additionally, IKK-16 is utilized in cellular and molecular biology experiments to dissect the role of NF-κB in various physiological and pathological processes. Its application extends to drug discovery, where it serves as a tool compound to develop novel treatments for diseases linked to NF-κB dysregulation.