BMS-345541 is primarily used in biochemical research as a selective inhibitor of the IκB kinase (IKK) complex, particularly targeting IKKβ. This compound is valuable in studying the NF-κB signaling pathway, which plays a critical role in inflammation, immune response, and cell survival. Researchers utilize BMS-345541 to investigate the effects of inhibiting this pathway in various disease models, such as cancer, autoimmune disorders, and chronic inflammatory conditions. Its ability to modulate NF-κB activity makes it a useful tool for understanding the molecular mechanisms underlying these diseases and for exploring potential therapeutic strategies. Additionally, BMS-345541 is employed in cell culture studies to assess its impact on gene expression, cytokine production, and apoptosis, providing insights into cellular responses to IKK inhibition.