ML-299 is primarily used in research settings to study its effects on the central nervous system. It acts as a potent and selective agonist for the trace amine-associated receptor 1 (TAAR1), making it a valuable tool for understanding the role of TAAR1 in neurophysiology and behavior. Researchers utilize ML-299 to explore its potential in modulating neurotransmitter systems, particularly dopamine and serotonin, which are implicated in various psychiatric and neurological disorders. Its application extends to preclinical studies aimed at investigating its therapeutic potential for conditions such as schizophrenia, depression, and addiction. Additionally, ML-299 is employed in pharmacological assays to elucidate the signaling pathways and mechanisms associated with TAAR1 activation, contributing to the development of novel therapeutic agents targeting this receptor.