TN-16 is primarily used as a research chemical in neuroscience for studying glycine receptors and their role in inhibitory neurotransmission. It acts as a selective antagonist at glycine receptors, making it a valuable tool for probing receptor function and synaptic mechanisms in the central nervous system. Due to its specificity, TN-16 is employed in in vitro and in vivo experiments to investigate neurological conditions such as epilepsy, spasticity, and chronic pain. It is also utilized in pharmacological studies aimed at developing new therapeutic agents targeting ion channels and receptor pathways. Its application remains confined to laboratory and experimental settings, with no current clinical use in humans.