Clozapine N-oxide is primarily used in research settings, especially in chemogenetic studies. It serves as an activator for engineered receptors known as DREADDs (Designer Receptors Exclusively Activated by Designer Drugs). When administered, it selectively binds to these receptors, enabling precise control over neuronal activity in specific brain regions. This application is valuable in neuroscience for studying neural circuits, behavior, and neurological disorders such as Parkinson’s disease, addiction, and epilepsy. Due to its ability to cross the blood-brain barrier and elicit targeted responses, Clozapine N-oxide is a key tool in preclinical models for understanding brain function and evaluating potential therapeutic interventions.