Nudifloside D is primarily studied for its potential anti-inflammatory and neuroprotective properties. It has shown activity in modulating pathways associated with oxidative stress, making it a candidate of interest in research on neurodegenerative conditions such as Alzheimer's and Parkinson’s disease. Its ability to cross the blood-brain barrier enhances its relevance in central nervous system therapeutics. Additionally, nudifloside D exhibits mild cytotoxic effects against certain cancer cell lines, suggesting possible applications in oncology, particularly as a lead compound for developing novel chemotherapeutic agents. Ongoing studies focus on its role in apoptosis induction and cell cycle regulation in tumor cells. Due to its natural origin and structural complexity, it is also used as a reference standard in phytochemical analysis of medicinal plants.