Pifithrin-μ is widely used in research for its ability to inhibit the mitochondrial pathway of apoptosis. It protects cells from stress-induced death by stabilizing the structure and function of mitochondria, making it valuable in studies related to neurodegenerative diseases, ischemia-reperfusion injury, and organ protection during transplantation. It has shown promise in reducing damage in models of stroke and heart attack by preventing mitochondrial permeability transition pore opening. Additionally, Pifithrin-μ is used to investigate cancer cell survival mechanisms, as it can modulate cellular responses to chemotherapeutic agents. Its role in enhancing cell viability under stress makes it a tool compound in developing therapies for conditions involving mitochondrial dysfunction.