Corticotropin-releasing factor (CRF), particularly the ovine variant, is primarily used in medical and research settings to study and understand the hypothalamic-pituitary-adrenal (HPA) axis. It is employed to stimulate the release of adrenocorticotropic hormone (ACTH) from the pituitary gland, which in turn triggers the adrenal glands to produce cortisol. This mechanism is crucial in research focused on stress responses, anxiety disorders, and depression. Additionally, ovine CRF is utilized in diagnostic tests to assess pituitary function and to identify abnormalities in the HPA axis. Its role in these applications helps in developing therapeutic strategies for conditions related to stress and hormonal imbalances.