N-terminally acetylated Endomorphin-1 is primarily used in research settings to study its interactions with opioid receptors, particularly the mu-opioid receptor. Its modified structure enhances stability and bioavailability, making it a valuable tool for investigating pain modulation pathways. Researchers utilize this peptide to explore its potential analgesic effects and to develop novel pain-relief therapies. Additionally, it serves as a model compound for studying peptide-receptor interactions and the role of acetylation in peptide activity and stability. Its applications extend to neuropharmacology, where it aids in understanding the mechanisms of opioid signaling and the development of targeted therapeutic agents.