Prionoid B is primarily used in research settings for studying protein misfolding and aggregation processes associated with neurodegenerative diseases. It serves as a molecular tool to induce and monitor prion-like behavior in cellular and biochemical models, helping scientists understand the mechanisms behind diseases such as Creutzfeldt-Jakob disease and other amyloid-related disorders. Its ability to promote conformational changes in proteins makes it valuable in assays designed to screen potential therapeutic compounds that inhibit or reverse abnormal protein aggregation. Due to its specificity in triggering cascade misfolding, Prionoid B is also employed in the development of diagnostic methods for early detection of prion diseases.