Org 27569 is primarily used in scientific research as a negative allosteric modulator of the cannabinoid receptor CB1. It is employed to study the function and signaling mechanisms of CB1 receptors in the central nervous system. Researchers use Org 27569 to investigate how modulation of CB1 activity affects behaviors related to addiction, appetite, pain, and mood regulation. Due to its ability to enhance binding of certain agonists while inhibiting downstream signaling, it serves as a valuable tool for understanding receptor dynamics and allosteric regulation. It is not used clinically and remains restricted to laboratory and experimental settings.