Used as a potent and selective antagonist of the lysosomal two-pore channel (TPC) family, particularly TPC2. It is widely applied in cellular and molecular research to investigate the role of TPCs in endolysosomal trafficking, autophagy, and calcium signaling. trans-Ned-19 helps modulate processes such as viral entry, where some pathogens exploit the endolysosomal system for host cell invasion. It has also been utilized in studies related to neurodegenerative diseases, lipid metabolism, and pigmentation due to TPC involvement in melanosome function. Its ability to inhibit NAADP-mediated calcium release makes it a valuable tool for probing NAADP-dependent physiological pathways.