ML-SI3 is primarily used as a selective agonist for TRPML3 (Transient Receptor Potential Mucolipin 3) channels in scientific research. It plays a key role in studying intracellular trafficking, lysosomal function, and membrane dynamics. Due to its ability to activate TRPML3 specifically, it is widely applied in cell biology and neuroscience to probe lysosomal ion channel activity and associated signaling pathways. ML-SI3 is also used to investigate the role of TRPML channels in diseases such as lysosomal storage disorders and neurodegenerative conditions. Its selectivity makes it a valuable tool for developing targeted therapies and understanding cellular homeostasis.