ML213 is primarily used in scientific research as a selective activator of the TRPM8 ion channel, which is involved in sensing cold temperatures. It is commonly applied in studies related to thermosensation and pain pathways, particularly those associated with cold hypersensitivity. Researchers use ML213 to investigate the physiological and pathological roles of TRPM8 in conditions such as neuropathic pain, migraine, and overactive bladder. Due to its ability to modulate channel activity, it serves as a valuable tool compound for validating TRPM8 as a therapeutic target and for screening potential drug candidates. Its application remains confined to preclinical research and is not used in clinical or industrial settings.