L-trans-Pyrrolidine-2,4-dicarboxylic acid is primarily used as a selective inhibitor of high-affinity glutamate transporters in neuroscience and pharmacological research. It blocks glutamate reuptake, facilitating studies on excitatory amino acid neurotransmission, synaptic plasticity, and related disorders such as epilepsy, schizophrenia, and neurodegeneration. This compound is employed in in vitro and in vivo models to investigate brain function and neurotransmitter signaling mechanisms. Additionally, its chiral pyrrolidine structure with dicarboxylic acid groups serves as a building block in organic synthesis for pharmaceuticals, enzyme inhibitors, proline analogs, and peptidomimetics, supporting asymmetric synthesis due to its stereochemical properties.