Used primarily in neuroscience research to study cholinergic systems. It inhibits the high-affinity choline transporter (CHT1), which is essential for acetylcholine synthesis in nerve terminals. By blocking choline uptake, it reduces acetylcholine production and release, allowing researchers to investigate the role of acetylcholine in synaptic transmission, memory, and neuromuscular function. Commonly applied in experimental models to induce cholinergic hypofunction, mimicking aspects of neurodegenerative diseases like Alzheimer’s. Also used to assess the functional capacity of cholinergic neurons in vitro and in vivo.