Used in research to inhibit gap junction communication between cells, particularly in studies related to cell signaling, tissue development, and cardiac function. Commonly applied in cardiovascular research to examine arrhythmias and the role of intercellular communication in heart tissue. Also utilized in neuroscience to investigate glial cell interactions and neuronal synchronization. Its ability to block connexin channels makes it a valuable tool for understanding cellular coordination in both healthy and diseased states. Often employed in in vitro models due to its specificity and reversible effects.