Guvacine is primarily used in scientific research, particularly in neuroscience and pharmacology. It acts as a selective inhibitor of GABA uptake, making it a valuable tool for studying the role of GABAergic neurotransmission in the central nervous system. By blocking the reabsorption of GABA, guvacine helps increase GABA concentration in synaptic clefts, allowing researchers to investigate the effects of enhanced inhibitory signaling. This property is useful in studying conditions such as epilepsy, anxiety, and other neurological disorders involving GABA imbalance. Additionally, guvacine serves as a structural analog in the development and testing of new compounds targeting GABA transporters. Its use remains largely confined to laboratory and experimental settings, with no widespread clinical or industrial applications currently established.