RO2959 hydrochloride is primarily utilized in research settings, particularly in the study of neurological and psychiatric disorders. It acts as a selective antagonist for certain receptors in the brain, making it valuable for investigating the mechanisms underlying conditions such as anxiety, depression, and schizophrenia. Researchers use this compound to explore the role of specific neurotransmitter systems in behavior and brain function. Additionally, it has potential applications in the development of novel therapeutic agents targeting these receptors, offering insights into new treatment strategies for mental health disorders. Its use is confined to preclinical studies, contributing to a better understanding of neuropharmacology.