Thalifoline is primarily used in pharmaceutical research due to its biological activity. It serves as a key intermediate in the synthesis of certain alkaloid-based drugs, particularly those targeting the central nervous system. Its structural properties make it valuable in developing compounds with sedative and anti-arrhythmic effects. It is also investigated for its potential anti-inflammatory and antioxidant applications in medicinal chemistry. Additionally, thalifoline is employed in studies exploring natural product biosynthesis and enzyme inhibition.