D-Allylglycine hydrochloride is primarily used in biochemical research as an inhibitor of the enzyme glutamate decarboxylase (GAD). By inhibiting GAD, it reduces the synthesis of gamma-aminobutyric acid (GABA), a key neurotransmitter in the central nervous system. This property makes it valuable in studies focused on understanding GABAergic signaling and its role in various neurological conditions, such as epilepsy, anxiety, and sleep disorders. Additionally, it is employed in experimental models to induce GABA deficiency, helping researchers explore the physiological and behavioral effects of altered GABA levels. Its application extends to investigating the mechanisms of action of potential therapeutic agents targeting the GABAergic system.